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Home >  Products >  Prizmatix IN-VIVO Optogenetics Toolbox >  Optogenetics-LED-Yellow

  Optogenetics-LED-Yellow

       USA/Canada: +1 (248) 436-8085 
 

 Fiber Coupled Yellow LED for Optogenetics in Freely Moving Mammals

 

 Introduction

The Prizmatix Optogenetics-LED-Yellow light source is specially designed to provide Yellow light (~595nm) for Optogenetics silencing in freely moving mammals. The Yellow light is especially fit Halorhodopsin (NpHR, eNpHR3.0) Yellow light inhibition opsin. This LED light source provides powerful light irradiance of >80 mW/mm² at fiber implant tip following all fiber-optic connections including Rotary Joint

Optogenetics-LED-Yellow
Optogenetics-LED-User-Manual

Optogenetics LED 460nm

 
 

 
 

 Key Features

  • High Power density (>80 mW/mm²) at fiber's tip following all connections
  • Extremely low torque LED-compatible rotary joint suitable for even the smallest animals.
  • Precisely adjustable power by 10 turns potentiometer
  • TTL and Analog Inputs are Opto-Isolated to eliminate ground-loops
  • Analog input (0-5V) for power control
  • USB control (optional)
  • Instant warm up time
 
 
 
 

 Optogenetics Toolbox for Freely Moving Mammals Experiments

Prizmatix  offers a wide range of standard and customized items comprising the Optogenetics Toolbox. The most useful items for optogenetics experiments  in freely  moving mammals are:

  1. Optogenetics-LED-Yellow
  2. Extremely low friction Rotary-Joint
  3. Fiber Optics Cannulae
  4. Single or Dual Fiber for bilateral activation
  5. Fiber patch cord
  6. Optional Pulser module with Windows software

Optogenetics-Toolbox-for-Free-Moving-Animals

For more information on items 2-6 please see here

 
 
 
 

 Optical Specifications

Wavelength nm 595
Wavelength range nm ±7
Spectrum half width nm 73
Optical Power from 200μm NA0.66 fiber (1m) mW >4
Optical Power from 250μm NA0.66 fiber (1m) mW >6
Optical Power from 500μm NA0.66 fiber (1m) mW >25
Optical Power from 1000μm NA0.63 fiber (1m) mW >100

Optical power and irradiance data for full Optogenetics system including Optogenetics LED Yellow, fiber patch cord, Rotary Joint, Dual / Single fiber and fiber optic cannula (implant):

Optical Power from 200μm cannula mW >2.5
Optical Power from 250μm cannula mW >4.5
Optical Power from 500μm cannula mW >13
Irradiance from 200μm cannula mW/mm² >80
 
  Optogenetics Toolbox for Free Moving Animals
  Optogenetics-LED-Yellow spectrum     Optogenetics-LED-Yellow Rise/Fall Time.  
   Ch1 – TTL input , Ch2         
Photodiode measurement
 

Idea!!!
Want to do implant (cannula) by yourself? We offer DIY cannula components such as high NA fibers and ceramic ferrules for online purchases. Please visit:
 
 
 
 

 Electrical Specifications

Digital modulation input   Optically isolated TTL
Digital modulation frequency Hz DC-30000
Rise / Fall time (10% - 90%) μs <3/ <10
Analog input voltage range V 0-5
Input Voltage V 12
Power Adaptor Input   85-264 VAC, 47-63Hz,1.5A
 
 

 
 

 General Specifications

Operation temperature range 0C 10-35
Storage Temperature range °C -10-35
Operating relative humidity
Non condensing
% <90
Dimensions (L x W x H) mm 197 x 174 x 80
Weight g 750
Power Adaptor Dimensions
(Lx W x H)
mm 125 x 50 x 31.5
Power Adaptor Weight g 300
Power Adaptor Safety  
Fan Noise dBA 28.4
 
 
 

 For more details on optional accessories please see:
 
 

 Rotary Joint

Prizmatix Rotary Joint is especially designed for Optogenetics experiments with High NA fibers equipped with FC connectors. Rotary Joint very low friction and smooth swivel operation are demonstrated in this video - showing continuous rotation for over 30 seconds after a fingertip startup. The torque required for free movement is very low thus reducing the Rotary Joint's behavioral effect on freely moving animals.
Prizmatix Rotary Joint can be used with large core input optical fiber connected to several thin output fibers simultaneously. This allows concurrent delivery of light to separate areas of the brain without loss of power due to the fiber split.
See more..

Rotary Joint PDF


Fiber-Optic-Rotary-Joint

 Optogenetics Implantable Cannulae

Prizmatix Implantable Fiberoptic Cannulae / Ferrules allow direct light stimulation of certain deep brain regions in living animals. Each Cannula consists of a Zirconia ferrule accommodating a high NA Silica optical fiber, protruding from the Zirconia ferrule at a desired length.
See more...


Cannula PDF


 Single/Dual Fibre

Prizmatix provides a full solution to the optogenetics in vivo and in vitro fiber optics. Made of silica or polymer fibers, the high NA fibers are assembled to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. Some examples are Y-shaped fibers for simultaneous in-vivo stimulation of two hemispheres, and fibers with stainless steel tip with protruding bare fibers for in-vitro activation of brain slices under a microscope. See more ...


Fiberoptics for Optogenetics PDF

Optogenetics-Protruding-Fiber-tip

  Fiber Patch Cords

Prizmatix offers a wide range of standard and customized multi-mode silica / polymer fibers as well as ferrules for optogenetics research. A Y-shaped fiber bundle is especially useful for activating two brain hemispheres simultaneously: a 1000μm or 1500μm fiber is coupled to the LED and connected to one side of the Rotary Joint. From the Joint’s other side emerge two thin fibers which can then be connected to two separate ferrules: the light from the LED is divided equally between the two hemispheres without any loss of power.


  Pulser

The Pulser USB to TTL interface box with user friendly software is an easy way to generate trains of  pulses for Optogenetics activation directly from your computer.   See more..

Pulser PDF


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