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Optogenetics-LED-Dual

                      USA/Canada: +1 (248) 436-8085
 

 Fiber Coupled Dual LED for Optogenetics in  Freely Moving Mammals

 Introduction

Designed for scaling Optogenetics experiments, Dual-Optogenetics-LED light source features two independent LED channels with independent power and switching control.

The wavelengths combination can suit widely used Stimulation and Silencing opsin pairs, such as ChR / ArchT, ChR / C1V1, ChR / EnpHR3.0 be Blue / Green, Blue / Yellow and Blue / Red and other wavelengths upon request.

Each channel of the Dual-Optogenetics-LED based system enables bilateral activation / inhibition for significant cost saving without compromising power at implant tip.
  Optogenetics-LED-Dual
  Optogenetics-LED-User-Manual

            Click picture to enlarge

 
 

Key Features

  • High Power density (at Blue >350 mW/mm²) at implant tip following Rotary Joint and all connections
  • Precisely adjustable power by 10-turns potentiometer
  • Isolated (optocoupler) TTL external modulation input
  • Analog input (0-5V) for power control
  • Instant warm up time
 

Optogenetics Toolbox for Freely Moving Animals Experiments

 

Prizmatix offers a wide range of standard and customized items comprising the Optogenetics Toolbox. The most useful items for optogenetics experiments  in freely  moving mammals are:

  1. Optogenetics-LED-Dual-Blue
  2. Extremely low friction Rotary-Joint
  3. Fiber Optics Cannulae
  4. Single or Dual Fiber for bilateral activation
  5. Fiber patch cord
  6. Pulser for generation of pulse train

                        Click picture to enlarge

For more information on items 2-6 please see Optional Accessories below

 
 

 Optical Specifications

 Following are the typical data for most common LED wavelengths:
 
Color nm Blue Green Red
Wavelength nm 460 520 625
Peak wavelength range nm 450-465 ±7 ±7
Spectrum half width nm 27 40 25
Optical Power from 200μm NA0.66 fiber (1m) mW >16 >7 >5
Optical Power from 250μm NA0.66 fiber (1m) mW >25 >10 >8
Optical Power from 500μm NA0.63 fiber (1m) mW >140 >40 >30
Optical Power from 1000μm NA0.63 fiber (1m) mW >600 >160 >120
 
Optical power and irradiance data for full Optogenetics system including Dual-
 
Optogenetics-LED, fiber patch cord, Rotary Joint, Dual / Single fiber
 
and fiber optic cannula (implant):
Optical Power from 200μm cannula mW >11 >4 >3
Optical Power from 250μm cannula mW >17 >5 >4.5
Optical Power from 500μm cannula mW >64 >20 >15
Irradiance from 200μm cannula mW/mm² >350 >120 >100
 
 
Idea!!!
Prizmatix‘s unique large emitter LEDs enable bilateral stimulation with a single low cost light source and simple one channel rotary joint. Using a simple Y-shaped fiber bundle at the rotary joint will double the amount of light delivered to the brain each branch delivering virtually the same power as a single fiber. This results in significant cost saving in setting up bilateral brain activation and silencing systems, compared to alternative light sources.

Idea!!!
Want to do implant (cannula) by yourself? We offer DIY cannula components such as high NA fibers and ceramic ferrules for online purchases. Please visit:
 
  Optogenetics Toolbox for Free Moving Animals
  Optogenetics-LED-Blue spectrum Optogenetics-LED-Blue Rise/Fall Time.
Ch1 TTL input, Ch2 Photodiode measurement
 
 

Electrical Specifications

Digital modulation input   Optically isolated TTL
Connector for TTL input   BNC
Digital modulation frequency Hz DC-30000
Rise / Fall time (10% - 90%) μs <3 / <10
Connector for Analog input   BNC
Analog input voltage range V 0-5
Analog modulation frequency Hz DC-100
Input Voltage V 12
Max Input Current A 6.5
Power Adaptor Input   85-264 VAC, 47-63Hz,1.5A
 

 

General Specifications

Operation temperature range °C 10-35
Storage Temperature range °C -10-35
Operating relative humidity
Non condensing
% <90
Dimensions (L x W x H) mm 197 x 174 x 80
Weight g 750
Power Adaptor Dimensions
(Lx W x H)
mm 175 x 72 x 35
Power Adaptor Weight g 650
Power Adaptor Safety  
Fan Noise dBA 28.4
 

 For more details on optional accessories please see:
 
 

 Rotary Joint

Prizmatix Rotary Joint is especially designed for Optogenetics experiments with High NA fibers equipped with FC connectors. Rotary Joint very low friction and smooth swivel operation are demonstrated in this video - showing continuous rotation for over 30 seconds after a fingertip startup. The torque required for free movement is very low thus reducing the Rotary Joint's behavioral effect on freely moving animals.
Prizmatix Rotary Joint can be used with large core input optical fiber connected to several thin output fibers simultaneously. This allows concurrent delivery of light to separate areas of the brain without loss of power due to the fiber split.
See more..

Rotary Joint PDF


Fiber-Optic-Rotary-Joint

 Optogenetics Implantable Cannulae

Prizmatix Implantable Fiberoptic Cannulae / Ferrules allow direct light stimulation of certain deep brain regions in living animals. Each Cannula consists of a Zirconia ferrule accommodating a high NA Silica optical fiber, protruding from the Zirconia ferrule at a desired length.
See more...


Cannula PDF


 Single/Dual Fibre

Prizmatix provides a full solution to the optogenetics in vivo and in vitro fiber optics. Made of silica or polymer fibers, the high NA fibers are assembled to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. Some examples are Y-shaped fibers for simultaneous in-vivo stimulation of two hemispheres, and fibers with stainless steel tip with protruding bare fibers for in-vitro activation of brain slices under a microscope. See more ...


Fiberoptics for Optogenetics PDF

Optogenetics-Protruding-Fiber-tip

  Fiber Patch Cords

Prizmatix offers a wide range of standard and customized multi-mode silica / polymer fibers as well as ferrules for optogenetics research. A Y-shaped fiber bundle is especially useful for activating two brain hemispheres simultaneously: a 1000μm or 1500μm fiber is coupled to the LED and connected to one side of the Rotary Joint. From the Joint’s other side emerge two thin fibers which can then be connected to two separate ferrules: the light from the LED is divided equally between the two hemispheres without any loss of power.


  Pulser

The Pulser USB to TTL interface box with user friendly software is an easy way to generate trains of  pulses for Optogenetics activation directly from your computer.   See more..

Pulser PDF


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