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UHP-Mic-LED-450 Ultra High Power LED Light Source
 for Optogenetics and Fluorescence Microscopy
 

 
 

Introduction

The Ultra High Power LED provides almost X10 increase of power in comparison with similar High power LED devices. The Ultra High Power LED ( > 36Watt) is an effective replacement of lasers and lamps in many power demanding applications, such as optogenetics, fluorescence microscopy, machine vision, chemical reaction activation and numerous others.

In Optogenetics the LED can be used for both in-situ and in-vivo applications. In in-situ experiments the LED can be connected to epifluorescence port of microscope, in in-vivo experiments the LED can be used with fiber coupler adaptor to enable opsin activation through fiber optic ferule in freely moving animals.

In fluorescence microscopy such markers as GFP, Quantum dots and other blue wavelength excitation markers can be used with this ultra-high power LED light source. The 450 nm LED will supply the needs of such power hungry methods as Photostimulation of Channelrhodopsin (ChR1, ChR2), FISH and FRAP.

This member of the modular Optiblocks family provides >2.5W of collimated LED power on its output and can be used with a full range of other optical OptiBlocks such as Fiber Coupler, Liquid Light Guide coupler, Beam Combiner, Filter Wheel, Beam Switcher and others.

Please see full list of Optional Accessories

See other Ultra-High-Power-LED wavelengths

Ultra-High-Power-LED-Microscope
 
UHP LED Fiber Coupled
 

 
 

Key features

  • Compatible with Prizmatix Optogenetics Toolbox and modular Microscope-LED Light Source products families
  • Single chip Ultra High Power LED
  • Collimated, Fiber Coupled or Light Guide options of light delivery
  • Optically isolated TTL input for external triggering
  • Analog input for light power control (optional)
  • Excellent for fluorescence excitation
  • LED spectrum can be narrowed by optional band pass filter installed inside the head
  • Stable precisely adjustable power
  • Long life (no lamp or laser tube replacement required)
  • Speckle free
  • Rapid warm up time
 
 

Applications

  • Optogenetics:Photostimulation of Channelrhodopsin ChR1, ChR2
  • Excitation of CFP, ECFP, Quantum Dots, Auramine, AmCyan, DiA
  • Whole body imaging
  • Photo activation (PA)
  • Fluorescence recovery after photo bleach (FRAP)
  • Fluorescence in-situ hybridization (FISH)
  • Bio analysis
  • Machine vision
 
 

Optical Specifications

Wavelength ±5 450
Wavelength range nm ±5
Spectrum FWHM nm 22
Collimated optical power output Watt >2.5
                         
 
 

Benchtop LED Controller Specifications

Features:

  • Constant current or chopping modes
  • Precise LED current setting by 10 turn dial
  • TTL external trigger input
  • Compact and robust enclosure
  
Digital modulation input Optically isolated TTL
Connector for TTL input BNC
Digital modulation frequency Hz DC-30000
Rise /Fall time (10%-90%) µs <10
Controller Input Voltage V 12
Controller Max Input current A 5.5
Power Adaptor Input 100-240 VAC, 50-60Hz,1.5A
 
 

General Specifications

 
 
  
Operation temperature range 0C 10-35
Storage temperature range 0C -10-55
Operative relative humidity (Non condensing) % <90
Head dimensions See drawing below
Head weight g 350
Controller dimensions (LxWxH) 220mm x 165mm x 51.5mm
Controller weight g 700
Power adaptor dimensions (LxWxH) 146mm x 76mm x 44mm
Power adaptor weight g 600
Fan noise dBA 38
 
 

Performance

 
 
UHP-Mic-LED-450 spectrum UHP-Mic-LED-450 stability
 
 

Mechanical drawings


 


Optional Accessories


 
Accessories
 

All Prizmatix's LED light sources can be easily configured for use in a wide variety of applications by simply adding further components. These use a standard 1.035” 40TPI thread (SM1 compatible) or Prizmatix's 4-pin connection system.

The following table summarizes the most popular accessories according to application, further information on individual part can be found below the table. Custom solutions are also often available at a low cost: please contact us with your specific requirements.

#

Accessories

Common Applications

 

Fiber delivery, Spectroscopy


Microscopy and Imaging


 

Optogenetics  in- vivo


Optogenetics  in-vitro

 1 Prizmatix Ultra High Power LED Light Source
           
 2 Beam Combiner – Combines several LEDs into one beam using a dichroic mirror
           
 3 FCA – Fiber Coupler Adaptor couples an optical fiber to LED: SMA or FC connectors            
 4 Fiber Patch Cord – Polymer / silica optical fiber (single or multiple branches), SMA or FC connectors            
 5 Rotary-Joint – Fiber optic Rotary Joint for in-vivo optogenetics            
 6 Beam Switcher – Changes direction of beam output (e.g. microscope to fiber)            
 7 Filter wheel – Takes up to six 1” filters            
 8 Microscope Adaptors - Adaptors for epi-fluorescence ports of Nikon / Olympus / Zeiss / Leica microscopes            
 9 LLG-A - Couples Liquid Light Guide to LED system            
10 LLG-3 / LLG-5 - Liquid Light Guide: 3mm or 5mm core            
11 LLG-C - Collimates Light Guide beam for fluorescent microscope            
12 C-Mount Adaptor - Used to mount LED on camera port of microscope            
13 Fiber Collimator – Specially designed to collimate high NA optical fibers            
14 Reference Photodiode - Monitors LED system power output            
15 Optogenetics-Fiber - For in-vivo applications, single or bilateral dual fiber            
16 Implantable Cannulae - Used with fiber patch cord in optogenetics            
17 Optogenetics-Fiber - For in-vitro applications: bare fiber protruding from thin stainless steel tubing            
18 Pulser – Programmable TTL pulse train generator            
19 USB-TTL Interface - To control Prizmatix LEDs from Imaging software (e.g. microManager) via USB connection            



 Prizmatix Ultra High Power LED Light Source [1]

Any type of Prizmatix Ultra High Power LEDs can be combined to form multi-wavelength system. For more information on specific types please see following links:  UHP-Mic-LED,  UHP-T-LED, Mic-LED

 Beam Combiner [2]

Beam Combiner module joins two discrete Prizmatix LEDs into one output beam using a built-in dichroic mirror at specified cut-off wavelength .
The beam combiner has additional port that can be used for connection of monitoring photodiode.

 FCA - Fiber Coupler Adaptor [3]

All Prizmatix LED heads can be easily changed from collimated LED source to fiber coupled LED configuration by means of Fiber Coupler Adaptor (SMA, FC or ST connector). .
For more details please see video clip

 Fiber patch cords [4]

Various fiber optic patch cords are available. Most popular patch cords are 1000 / 1500 / 2000
micron core diameter Polymer Optical Fibers (POF) terminated by optical SMA or FC connectors.
For Optogenetics applications Dual or Triple Y shape bundles can be useful for multi-site activation.
For spectroscopy application special fiber optic bundles are very important. Care must be addressed to eliminate possible cross link between the excitation and the collection fibers. See more info here and here .

 Rotary-Joint [5]

Prizmatix Rotary Joint is especially designed for Optogenetics experiments with High NA fibers equipped with FC connectors. Due to very low friction and smooth swivel the rotary joint is suitable for use even with small mice. See more info...

 Beam Switcher [6]

The Beam Switcher accessory allows for Prizmatix LEDs installed on a microscope to be used either as microscope epi-fluorescence illumination or illuminating a specimen via a fiberoptic probe this feature especially useful for in-situ Optogenetics studies. See more info...

 Filter Wheel [7]

The manual 6 positions filter wheel can be especially useful for White LED sources. The Filter Wheel can be installed at the beam output of LED or a Beam Combiner. See more info...

 Microscope Adaptors [8]

Adaptors enabling connection of Prizmatix LED light sources to epi-fluorescence light port. Adaptors for Nikon, Zeiss, Olympus and Leica microscopes are available. See more info...

 LLG-A Liquid Light Guide Adaptor [9]

The LED system can be quickly and easily attached to Liquid Light Guide by means of LLG-A Adaptor. For more details please see video clip

 LLG-3 / LLG-5 [10]

The Liquid Light Guide is a common way to connect light source to microscope epi-fluorescence port. Prizmatix can provide 3mm or 5mm core LLGs.

 LLG-C collimator XYZ [11]

The Prizmatix LLG collimator is an XYZ adjustable collimator for 3mm core Liquid Light Guide (LLG-3) with adaptor for the epi-illumination port of fluorescence microscopes. This collimator can be equipped with Nikon, Olympus, Zeiss or Leica adaptors. See more info...

 C-Mount Adaptor [12]

A standard C-mount thread adaptor (1-32 UN 2A thread), usually used to connect to trinocular microscopes.

 Collimator [13]

The output from optical fiber is divergent relative to fiber NA. In order to reduce the divergence angle a collimator module can be used. Prizmatix's collimator is especially designed to fit thick core high NA polymer optical fibers. See more info...

 Reference Photodiode [14]

Prizmatix reference photodiode is useful in order to monitor LED source power over time in experiments required exceptional stability over long time period.

 Optogenetics-Fiber for in-vivo [15]

Prizmatix provides a full solution to the optogenetics in-vivo and in-vitro fiber optics. Made of silica or polymer high NA fibers, the high NA fibers are assembled to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. This is example of a Y-shaped fiber for simultaneous in-vivo stimulation of two hemispheres. See more info...

 Implantable Cannulae [16]

Prizmatix Implantable Fiberoptic Cannulae / Ferrules allow direct light stimulation of certain deep brain regions in living animals. Each Cannula consists of a Zirconia ferrule accommodating a high NA Silica optical fiber, protruding from the Zirconia ferrule at a desired length. See more info...

 Optogenetics-Fiber for in-vitro [17]

Prizmatix provides a full solution to the optogenetics in-vivo and in-vitro fiber optics. Made of silica or polymer fibers, the high NA fibers are assembled to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. This is example of a fiber with stainless steel tip with protruding bare fiber for in-vitro activation of brain slices under a microscope. See more info...

 Pulser [18]

The Pulser USB to TTL interface box with user friendly software is an easy way to generate trains of pulses for Optogenetics activation directly from your computer. See more info...

 USB-TTL Interface [19]

The microprocessor based device enabling simple control of Prizmatix LED sources from Windows based computer via USB.


 Application Examples:


 Optogenetics  LED Light Source:

Optogenetics illumination sources include lasers and LEDs, and photoactivation can be done under a microscope or via a fiber for in-vitro or in-vivo applications. Using a Prizmatix LED illumination system for optogenetics studies provides many advantages compared to laser-based systems.
Read more... 

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