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Optogenetics Implantable Cannula for in-vivo applications

 Introduction

Prizmatix Implantable Fiberoptic Cannula developed for optogenetics allows direct stimulation of target deep brain tissues in freely moving animals.

Each Cannula consists of a Zirconia ferrule accommodating a high NA Silica optical fiber, protruding from Zirconia ferrule at desired length. The fiber is optically polished both ends to ensure high quality optical coupling and reliability.

All cannula components are biocompatible, implantable and non-metallic, making them NMR/MRI compatible.

Standard and custom built cannulae are also available in two ferrules diameters: 2.5mm and lightweight 1.25mm for smaller animals as mice.

Zirconia Mating Sleeves for these cannulae are available from Prizmatix

 Optogenetics Cannula



Optogenetics Cannula with sleeve and fiber


Optogenetics Cannula with sleeve

Key Features

  • Zirconia ferrules - 2.5mm or lightweight 1.25mm outer diameter e.g. for mice.
  • High NA fibers – 200μ (NA 0.66), 250μ (NA 0.66), 400μ core (NA 0.53).
  • Biocompatible and Implantable
  • Adheres to the cement fixing the cannula to the skull.
  • NMR compatible, no metallic components.
  • Polished ends.
  • Sterilizable - alcohol, ETO, autoclave (65°C / 1 hour cure).
  • Electrically isolated.
  • Any required fiber length.
  • A special custom Peripheral Nerve Implants are available. Please contact us.

Applications

  • In-vivo Optogenetics in behaving animals

Specifications


Wavelength range   - 350nm - 1500nm


Optogenetics Cannulae with sleeves
Fiber Length protruding from cannula:   
Fiber Core Diameter
Fiber Outer Diameter
 NA  Protruding (mm)
400μm 470μm 0.53 0.5 < L< 15
250μm 275μm 0.66 0.15 < L< 15
200μm 225μm 0.66 0.5 < L< 15

Example of pulsed output from Prizmatix LED-460 after rotary joint:   
Fiber Core Diameter
Fiber Outer Diameter
 NA  Power Irradiance
400μm 470μm 0.53 >40mW >320mW/mm²
200μm 225μm 0.66 >10mW >320mW/mm²

Example of pulsed output from Prizmatix LED-520 after rotary joint:   
Fiber Core Diameter
Fiber Outer Diameter
 NA  Power Irradiance
400μm 470μm 0.53 >16mW >120mW/mm²
200μm 225μm 0.66 >4mW >120mW/mm²
 

Idea!!!
Want to do implant (cannula) by yourself? We offer DIY cannula components such as high NA fibers and ceramic ferrules for online purchases. Please visit:


Zirconia Ferrule:   
  Diameter
  2.5mm             1.25mm
Zirconia ferrule Length mm 11.4 6.8
Sleeve Pull Force N 1.5 - 4.5 1.0 - 2.9
Cannula typical weight gram < 0.5  < 0.1 


Mechanical Drawings

Optogenetics-Cannula-Drawing

  • L - Fiber length protruding from cannula. See specification table for details.
  • Zirconia Mating sleeves for these cannulae are available from Prizmatix.

 Additional information

Prizmatix Canuulae are specially designed for working with high NA fibers and LED light sources Unlike lasers, Prizmatix Ultra High power LEDs have a high brightness large emitting area and wide angle radiance

We use this characteristic to couple the LED's light efficiency into a high numerical aperture (NA ≥0.5) primery large core fiber, e.g. 1000μm - 1500μm core. Due to the large core of the primery fiber, coupling of the secondary fiber (NA≥0.5) with smaller core, e.g. 500μm, does not require precise axial alignment.Having the fibers close enough to each other, just keeping the small core in front of the larger core -it keeps efficient light coupling.

With Prizmatix Rotary Joint for in-vivo experiments, the primary fiber is connected to the top port of fiberoptic rotary joint and the secondary fiber is connected to to the bottom of the rotary joint. Prizmatix Rotary Joint consists of a special low-friction ball-bearing which enables the two fibers to rotate freely upon each other.

The brightness, usually measures in mW/mm², is basically conserved along the fiberoptic chain from the LED light source towards the rotary joint and the implanted cannula, provided the Numerical Aperture (NA) is preserved and despite the fact that core diameter of the fiber optic components in the system get smaller.

The large core to small core(s) coupling method allows the secondary fiber to have more than one branch, still using a single LED light source and without compromising the brightness. This is very useful for comprehensive research, such as optogenetically manipulation of two brain hemispheres or at several locations simultaneously - see figure below.

Prizmatix Rotary Joint is suitable for systems based on LEDs and high NA fibers as Prizmatix offer in the Optogenetics Toolbox The high NA-Cannulae are the last component in this fiberoptic chain, exploiting the most of Prizmatix optogenetics LEDs.



 Optional Accessories

 High Poweer LEDs

The UHP-Mic-LED is a Ultra High Power LED light source for fluorescence microscopy and other applications.The UHP-Mic-LEd light source module is an effective replacement of special lamps and lasers in many applications.The LED driver supports CW or external TTL modulation with user-controlled frequency and duty cycle.For more details please click here

 Fiber Coupler Adaptor

The side port of the Beam switcher can be easily configured to fiber coupling by means of the FCA Fiber Coupler Adaptor (SMA, CF or ST connector). This adaptor can be easily assembled by a user on the Mic-LED output. Please see  video clip

 Rotary Joint

Prizmatix Rotary Joint is especially designed for Optogenetics experiments with high NA fibers equipped with FC connectors. Rotary Joint very low-friction ans smooth svivel operation are demonstrated in this  video clip showing continuous rotation for over 30 seconds after a fingertip startup. The torque required for free movement is very low thus reducing the Rotary Joint's behavioural effect on free moving animals.

Prizmatix Rotary Joint can be used with large core fiber input optical fiber connected to several thin output fibers simultaneously.This allows concurrent delivery of light to separate areas of brain without loss of power due to the fiber split. For more details please click here

 Fiber Bundles

To combine outputs of multiple LEDs a Y-shaped fiber bundle with two or more input branches can be used.Prizmatix can help to configure and build custom fiber bundles for specific applications.  For more details please click here

Optogenetics Componenets

 

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