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TitleWide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing
AuthorM Szalkowski, K Sulowska, J Grzelak, E. Rozniecka, D. Kowalska, S. Mackowski
Affiliation(s)1. Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, Poland 2 Baltic Institute of Technology, Al. Zwycięstwa 96/98, 81-451 Gdynia, Poland 3 Institute of Physical Chemistry Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224 Warsaw, Poland
Published Sensors, 2018 18(1), 290; https://doi.org/10.3390/s18010290
Keywordphotobleching . LED 405 nm
Snippet Excitation (λ = 405 nm, FWHM = 11.49 nm, P = 40 µW) was provided by a LED illuminator (Prizmatix Ltd.,
AbstractWe apply wide-field fluorescence microscopy to measure real-time attachment of photosynthetic proteins to plasmonically active silver nanowires. The observation of this effect is enabled, on the one hand, by sensitive detection of fluorescence and, on the other hand, by plasmonic enhancement of protein fluorescence. We examined two sample configurations with substrates being a bare glass coverslip and a coverslip functionalized with a monolayer of streptavidin. The different preparation of the substrate changes the observed behavior as far as attachment of the protein is concerned as well as its subsequent photobleaching. ///

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