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TitleMiniSOG-mediated Photoablation in Caenorhabdtis elegans
AuthorYingchuan Qi, Suhong Xu
Affiliation(s)Division of Biological Sciences, Neurobiology Section, University of California, San Diego, CA, USA
Publishedbio-protocol Vol 3, Iss 1, January 05, 2013 DOI: 10.21769/BioProtoc.316
KeywordUHP-Mic-LED-460
Snippet 
AbstractAbstract This protocol describes a method for light-inducible cell ablation in live worms. miniSOG (mini Singlet Oxygen Generator) generates singlet oxygen upon blue light illumination (Shu et al., 2011). Mitochondrially membrane targeted miniSOG (the first 55 a. a. of C. e. tomm-20 fused at the N’-terminus of miniSOG, termed as mito-miniSOG in the following) is transgenically expressed in specific cells/tissues (Qi et al., 2012). Groups of transgenic animals are illuminated under open field fluorescence light on a compound microscope or LED light setup for photo-ablation. Equipment Fluorescence stereomicroscope (dissecting microscope, e.g., Leica, model: M165 ) Upright epi-fluorescecne microscope (compound microscope, e.g., Zeiss Axio Imager 2) Prizmatix LED (UHP-Mic-LED-460), and Digital Function Generator (PASCO Scientific, catalog number: PI-9587C ) Microinjection rig to generate transgenic worms

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