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Optogenetics-LED-STSI

Fiber Coupled LEDs for Optogenetics experiments in Freely Moving Mammals

Introduction

The Prizmatix Optogenetics-LED-STSI module is specially designed to provide Optogenetics Stimulation / Silencing to the same neural structure in freely moving mammals. The device combines two LEDs of different colors into the same single output fiber. Each LED has independent power and switching control.

The wavelengths combination can suit widely used Stimulation and Silencing opsin pairs, such as ChR2 / ArchT,  ChR2 / C1V1, ChR2 / EnpHR3.0. These opsins pairs can be stimulated / silenced by Blue / Green,  Blue / Yellow and  Blue / Orange-Red wavelengths. Other wavelengths are available upon request.

The Optogenetics-LED-STSI based system optionally enables bilateral activation / inhibition with single device and single one-channel rotary joint for significant cost saving and without compromising power at implant tip.

Optogenetics-LED-STSI

Optogenetics-LED-User-Manual

      Click to enlarge



 Key Features

  • High Power density (>240 mW/mm²) at cannula's fiber tip following all fiberoptics connections and rotary joint.
  • Unique large and powerful LEDs enable bilateral illumination with single LED and rotary joint for significant cost saving and without compromising power at implant tip.
  • Extremely low torque LED-compatible rotary joint suitable for even the smallest animals
  • Precisely adjustable power by 10 turns potentiometers, or electronically controlled by Analog input ports (0-5V)
  • TTL and Analog Inputs are Opto-Isolated to eliminate ground-loops
  • Instant warm up time. Rise / Fall time <10 μs



Optogenetics Toolbox for Freely Moving Mammals Experiments

Prizmatix offers a wide range of standard and customized items comprising the Optogenetics Toolbox. The most useful items for optogenetics experiments in free-moving animals are:

  1. Optogenetics-LED-STSI
  2. Extremely low friction Rotary-Joint
  3. Fiber Optics Cannulae
  4. Single or Dual Fiber for bilateral activation
  5. Fiber patch cord
  6. Optional Pulser module with Windows software

For more information on items 2-6 please see Optional Accessories below

Optogenetics-Toolbox-for-Free-Moving-Animals

Common LED colors:

Opsin LED Color Peak wavelength range (nm)
  UV- Violet 385 - 410
ChR Blue 450 - 465
ArchT Green 515 - 525
C1V1 Green 515 - 525
Enphr3.0 Orange-Red 610 - 630

Optical Specifications

LED specifications Units Channel #1 Channel #2
Spectrum FWHM nm
Optical ouput power values from optical fiber connected directly to Optogenetics-LED.
Useful for in-vitro or fixed preparate applications:
Optical output power values from cannula of full Optogenetics system comprising Optogenetics-LED, fiber patch cord, Rotary-Joint, Dual/Single fiber and cannula inplant:

 Output Data      λ = 0 Channel #1 = 0 Channel #2 = 0

Wavelength (nm)                 


 Electrical Specifications

Digital modulation input   Optically isolated TTL
Connector for TTL and Analog input   BNC
Digital modulation frequency Hz DC-30000
Rise / Fall time (10% - 90%) μs <10
Analog input voltage range V 0-5
Input Voltage V 12
Power Adaptor Input   85-264 VAC, 47-63Hz,1.5A


 General Specifications

Operation temperature range °C 10-35
Storage Temperature range °C -10-35
Operating relative humidity
Non condensing
% <90
Dimensions (L x W x H) mm 197 x 174 x 80
Weight g 750
Power Adaptor Dimensions
(L x W x H)
mm 175 x 72 x 35
Power Adaptor Weight g 270
Power Adaptor Safety  
Fan Noise dBA 28.4


Optional Accessories

   For more details on optional accessories please see:
 

Rotary Joint

Prizmatix Rotary Joint is especially designed for Optogenetics experiments with High NA fibers equipped with FC connectors. Rotary Joint very low friction and smooth swivel operation are demonstrated in this video - showing continuous rotation for over 30 seconds after a fingertip startup. The torque required for free movement is very low thus reducing the Rotary Joint's behavioral effect on freely moving animals.
Prizmatix Rotary Joint can be used with large core input optical fiber connected to several thin output fibers simultaneously. This allows concurrent delivery of light to separate areas of the brain without loss of power due to the fiber split.
See more..

Rotary Joint


Fiber-Optic-Rotary-Joint

Optogenetics Implantable Cannulae

Prizmatix Implantable Fiberoptic Cannulae / Ferrules allow direct light stimulation of certain deep brain regions in living animals. Each Cannula consists of a Zirconia ferrule accommodating a high NA Silica optical fiber, protruding from the Zirconia ferrule at a desired length.
See more...

Cannula


Single/Dual Fibre

Prizmatix provides a full solution to the optogenetics in vivo and in vitro fiber optics. Made of silica or polymer fibers, the high NA fibers are assembled to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. Some examples are Y-shaped fibers for simultaneous in-vivo stimulation of two hemispheres, and fibers with stainless steel tip with protruding bare fibers for in-vitro activation of brain slices under a microscope.
See more


Fiberoptics for Optogenetics

Optogenetics-Protruding-Fiber-tip

Fiber Patch Cords

Prizmatix offers a wide range of standard and customized multi-mode silica / polymer fibers as well as ferrules for optogenetics research. A Y-shaped fiber bundle is especially useful for activating two brain hemispheres simultaneously: a 1000μm or 1500μm fiber is coupled to the LED and connected to one side of the Rotary Joint. From the Joint’s other side emerge two thin fibers which can then be connected to two separate ferrules: the light from the LED is divided equally between the two hemispheres without any loss of power.


Pulser

The Pulser USB to TTL interface box with user friendly software is an easy way to generate trains of  pulses for Optogenetics activation directly from your computer.   See more..

Pulser