Fiber Coupled Violet LED for Optogenetics in Freely Moving Mammals
Introduction
The Prizmatix Optogenetics-LED-Violet module is specially designed
to provide high power Violet light (~405nm) to activate various Opsins
with Violet activation spectrum in Optogenetics experiments with freely moving mammals.
This LED source provides powerful light irradiance of >380 mW/mm² at fiber implant tip
following all fiber-optic connections including Rotary Joint.
High Power density (>380 mW/mm²) at cannula's fiber tip following all connections
Unique large LED enables bilateral illumination with single LED and rotary joint for significant
cost saving and without compromising power at implant tip.
Extremely low torque LED-compatible rotary joint suitable for even the smallest animals
Precisely adjustable power by 10 turns potentiometer
TTL and Analog Inputs are Opto-Isolated to eliminate ground-loops
Analog input (0-5V) for power control
USB control (optional)
Instant warm up time
Some research papers which reference Prizmatix's Violet LED
1. Photoconversion of Alloreactive T Cells in Murine Peyer’s Patches During Acute Graft-Versus-Host Disease: Tracking the Homing Route of Highly Proliferative Cells In Vivo
The regulation of immune cell migration throughout the body is essential to warrant immunosurveillance
and to maintain immune homeostasis. Marking and tracking of these cells has proven important to study
mechanisms of immune cell trafficking and cell interaction in vivo. Photoconversion is a well-suited
technique for intravital application because it enables contactless time- and location-specific
marking of cells in the tissue without surgically manipulating the microenvironment of the cells
in question. However, in dividing cells the converted fluorescent protein may decline quickly.
Here, we provide a detailed description of the photoconversion technique and its applicability
to tracking highly proliferating T cells from the priming site of T cell activation to peripheral
target organs of effector function in a preclinical model. Dendra2+ T cells were photoconverted
in the Peyer’s patches during the initiation phase of acute graft-versus-host disease (GvHD) and
tracked through the mesenteric lymph nodes and the peripheral blood to the small intestine with
flow cytometry and intravital two-photon microscopy. Photoconverted alloreactive T cells preserved
the full proliferative capacity, homing, and migration of alloreactive T cells in the
intestinal lamina propria......
2. Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing
We apply wide-field fluorescence microscopy to measure real-time attachment of
photosynthetic proteins to plasmonically active silver nanowires. The observation
of this effect is enabled, on the one hand, by sensitive detection of fluorescence
and, on the other hand, by plasmonic enhancement of protein fluorescence.
We examined two sample configurations with substrates being a bare glass coverslip
and a coverslip functionalized with a monolayer of streptavidin. The different
preparation of the substrate changes the observed behavior as far as attachment
of the protein is concerned as well as its subsequent photobleaching.
Optogenetics Toolbox for Freely Moving Mammals Experiments
Prizmatix offers
a wide range of standard and customized items comprising
the Optogenetics Toolbox. The most useful items for
optogenetics experiments in free-moving animals are:
Optical power and irradiance data
for full Optogenetics system including Optogenetics-LED-Violet,
fiber patch cord, Rotary Joint, Dual / Single fiber and fiber optic cannula (implant):
Optical Power from 200μm cannula
mW
>12
Optical Power from 250μm cannula
mW
>18
Optical Power from 500μm cannula
mW
>66
Irradiance from 200μm cannula
mW/mm²
>380
Idea!!!
Prizmatix‘s unique large emitter LEDs enable
bilateral stimulation with a single low cost light
source and simple one channel rotary joint.
Using a simple Y-shaped fiber bundle at the rotary joint
will double the amount of light delivered to the brain
each branch delivering virtually the same power as a single fiber.
This results in significant cost saving in setting up bilateral
brain activation and silencing systems, compared to alternative light sources.
Idea!!!
Want to do implant (cannula) by yourself?
We offer DIY cannula components
such as high NA fibers and ceramic ferrules for online purchases.
Please visit:
Prizmatix's Rotary Joint has very low torque and friction,
with minimal impact on behavior. Prizmatix's Rotary Joint is
specially designed for Optogenetics experiments with
High NA detachable fibers equipped with FC connectors.
Rotary Joint can be used with single or multiple output
fibers simultaneously, enabling concurrent delivery of light
to separate areas of the brain without loss of power or brightness.
For more details please see: https://www.prizmatix.com/Optogenetics/Rotary-Joint.htm
Prizmatix Implantable Fiberoptic Cannulae / Ferrules allow
direct light stimulation of certain deep brain regions in living animals.
Each Cannula (implant) consists of a zirconia ferrule accommodating
a high NA Silica/POF fiber, protruding from the zirconia ferrule at
the specified length. For more details please see: https://www.prizmatix.com/Optogenetics/OG-Cannulae.aspx
Prizmatix provides diverse fiber optics solutions
for Optogenetics in-vivo. Made of silica, glass or polymer,
these high NA fibers can be constructed to fit any research
set-up with various combinations of connectors, ferrules,
core diameters and lengths. Examples include single
stainless steel coverings of bare tipped fibers,
or Y-shaped fibers for simultaneous stimulation of two hemispheres.
For more details please see: https://www.prizmatix.com/Optogenetics/Fiberoptics-for-Optogenetics.htm
The Pulser - USB pulse train generator with
user friendly software is an easy way to generate trains
of pulses for Optogenetics activation directly from your computer.
The Trigger-In input enables synchronization of
Optogenetics activation with various experimental events.
For more details please see:
https://www.prizmatix.com/Optogenetics/PulserPlus.htm
