The Prizmatix Optogenetics-LED-Violet module is specially designed
to provide high power Violet light (~405nm) to activate various Opsins
with Violet activation spectrum in Optogenetics experiments with freely moving mammals.
This LED source provides powerful light irradiance of >380 mW/mm² at fiber implant tip
following all fiber-optic connections including Rotary Joint.
High Power density (>380 mW/mm²) at cannula's fiber tip following all connections
Unique large LED enables bilateral illumination with single LED and rotary joint for significant
cost saving and without compromising power at implant tip.
Extremely low torque LED-compatible rotary joint suitable for even the smallest animals
Precisely adjustable power by 10 turns potentiometer
TTL and Analog Inputs are Opto-Isolated to eliminate ground-loops
Analog input (0-5V) for power control
USB control (optional)
Instant warm up time
Some research papers which reference Prizmatix's Violet LED
1. Photoconversion of Alloreactive T Cells in Murine Peyer’s Patches During Acute Graft-Versus-Host Disease: Tracking the Homing Route of Highly Proliferative Cells In Vivo
The regulation of immune cell migration throughout the body is essential to warrant immunosurveillance
and to maintain immune homeostasis. Marking and tracking of these cells has proven important to study
mechanisms of immune cell trafficking and cell interaction in vivo. Photoconversion is a well-suited
technique for intravital application because it enables contactless time- and location-specific
marking of cells in the tissue without surgically manipulating the microenvironment of the cells
in question. However, in dividing cells the converted fluorescent protein may decline quickly.
Here, we provide a detailed description of the photoconversion technique and its applicability
to tracking highly proliferating T cells from the priming site of T cell activation to peripheral
target organs of effector function in a preclinical model. Dendra2+ T cells were photoconverted
in the Peyer’s patches during the initiation phase of acute graft-versus-host disease (GvHD) and
tracked through the mesenteric lymph nodes and the peripheral blood to the small intestine with
flow cytometry and intravital two-photon microscopy. Photoconverted alloreactive T cells preserved
the full proliferative capacity, homing, and migration of alloreactive T cells in the
intestinal lamina propria......
2. Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing
We apply wide-field fluorescence microscopy to measure real-time attachment of
photosynthetic proteins to plasmonically active silver nanowires. The observation
of this effect is enabled, on the one hand, by sensitive detection of fluorescence
and, on the other hand, by plasmonic enhancement of protein fluorescence.
We examined two sample configurations with substrates being a bare glass coverslip
and a coverslip functionalized with a monolayer of streptavidin. The different
preparation of the substrate changes the observed behavior as far as attachment
of the protein is concerned as well as its subsequent photobleaching.
Optogenetics Toolbox for Freely Moving Mammals Experiments
Prizmatix offers
a wide range of standard and customized items comprising
the Optogenetics Toolbox. The most useful items for
optogenetics experiments in free-moving animals are:
Optical power and irradiance data
for full Optogenetics system including Optogenetics-LED-Violet,
fiber patch cord, Rotary Joint, Dual / Single fiber and fiber optic cannula (implant):
Optical Power from 200µm cannula
mW
>12
Optical Power from 250µm cannula
mW
>18
Optical Power from 500µm cannula
mW
>66
Irradiance from 200µm cannula
mW/mm²
>380
Idea!!!
Prizmatix‘s unique large emitter LEDs enable
bilateral stimulation with a single low cost light
source and simple one channel rotary joint.
Using a simple Y-shaped fiber bundle at the rotary joint
will double the amount of light delivered to the brain
each branch delivering virtually the same power as a single fiber.
This results in significant cost saving in setting up bilateral
brain activation and silencing systems, compared to alternative light sources.
Idea!!!
Want to do implant (cannula) by yourself?
We offer DIY cannula components
such as high NA fibers and ceramic ferrules for online purchases.
Please visit:
Prizmatix Rotary Joint is
especially designed for Optogenetics experiments
with High NA fibers equipped with FC connectors.
Rotary Joint very low friction and smooth swivel operation are demonstrated
in this video - showing continuous rotation for over 30 seconds after a
fingertip startup.
The torque required for free movement is very low thus reducing
the Rotary Joint's behavioral effect on freely moving animals.
Prizmatix Rotary Joint can be used with large core input optical fiber connected
to several thin output fibers simultaneously. This allows concurrent delivery of light
to separate areas of the brain without loss of power due to the fiber split. See more..
Prizmatix Implantable Fiberoptic Cannulae / Ferrules
allow direct light stimulation of certain deep brain regions
in living animals.
Each Cannula consists of a Zirconia ferrule
accommodating a high NA Silica optical fiber, protruding from
the Zirconia ferrule at a desired length. See more...
Prizmatix provides a full solution to the optogenetics
in vivo and in vitro fiber optics. Made of silica or polymer fibers,
the high NA fibers are assembled to fit any research set-up with various
combinations of connectors, ferrules, core diameters and lengths.
Some examples are Y-shaped fibers for simultaneous in-vivo
stimulation of two hemispheres, and fibers with stainless steel tip
with protruding bare fibers for in-vitro activation of brain
slices under a microscope. See more
Prizmatix offers a wide range of standard and
customized multi-mode silica / polymer fibers as well
as ferrules for optogenetics research.
A Y-shaped fiber bundle is especially useful
for activating two brain hemispheres simultaneously:
a 1000μm or 1500μm fiber is coupled to the LED and
connected to one side of the Rotary Joint.
From the Joint’s other side emerge two thin fibers which can
then be connected to two separate ferrules: the light from
the LED is divided equally between the two hemispheres
without any loss of power.
Optogenetics Pulser is a programmable TTL pulse train generator for pulsing LEDs, lasers and shutters used in Optogenetics activation. The Pulser device comes with user friendly software that enables easy visual setup of numerous pulse train configurations.
See more..