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 Optogenetics-LED-Violet

Fiber Coupled Violet LED for Optogenetics in Freely Moving  Mammals

Introduction

The Prizmatix Optogenetics-LED-Violet module is specially designed to provide high power Violet light (~405nm) to activate various Opsins with Violet activation spectrum in Optogenetics experiments with freely moving mammals. This LED source provides powerful light irradiance of >380 mW/mm² at fiber implant tip following all fiber-optic connections including Rotary Joint.

Optogenetics-LED-Violet


Optogenetics-LED-User-Manual

Optogenetics LED 460nm  

 Key Features

  • High Power density (>380 mW/mm²) at cannula's fiber tip following all connections
  • Unique large LED enables bilateral illumination with single LED and rotary joint for significant cost saving and without compromising power at implant tip.
  • Extremely low torque LED-compatible rotary joint suitable for even the smallest animals
  • Precisely adjustable power by 10 turns potentiometer
  • TTL and Analog Inputs are Opto-Isolated to eliminate ground-loops
  • Analog input (0-5V) for power control
  • USB control (optional)
  • Instant warm up time
 

 Optogenetics Toolbox for Freely Moving Mammals Experiments

 

Prizmatix offers a wide range of standard and customized items comprising the Optogenetics Toolbox. The most useful items for optogenetics experiments in free-moving animals are:

  1. Optogenetics-LED-Violet
  2. Extremely low friction Rotary-Joint
  3. Fiber Optics Cannulae
  4. Single or Dual Fiber for bilateral activation
  5. Fiber patch cord
  6. Optional Pulser module with Windows software
Optogenetics-Toolbox-for-Free-Moving-Animals

    For more information on items 2-6 please see Optional Accessories section below


Optical Specifications

Wavelength nm 405
class="intd1">Peak wavelength range nm 402-412
Spectrum half width nm 27
Optical Power from 200μm NA0.66 fiber (1m) mW >18
Optical Power from 250μm NA0.66 fiber (1m) mW >24
Optical Power from 500μm NA0.63 fiber (1m) mW >120
Optical Power from 1000μm NA0.63 fiber (1m) mW >480

Optical power and irradiance data for full Optogenetics system including Optogenetics-LED-Violet, fiber patch cord, Rotary Joint, Dual / Single fiber and fiber optic cannula (implant):

Optical Power from 200μm cannula mW >12
Optical Power from 250μm cannula mW >18
Optical Power from 500μm cannula mW >66
Irradiance from 200μm cannula mW/mm² >380
 

Idea!!!
Prizmatix‘s unique large emitter LEDs enable bilateral stimulation with a single low cost light source and simple one channel rotary joint. Using a simple Y-shaped fiber bundle at the rotary joint will double the amount of light delivered to the brain each branch delivering virtually the same power as a single fiber. This results in significant cost saving in setting up bilateral brain activation and silencing systems, compared to alternative light sources.

Idea!!!
Want to do implant (cannula) by yourself? We offer DIY cannula components such as high NA fibers and ceramic ferrules for online purchases. Please visit:
 
Optogenetics Toolbox for Free Moving Animals  
Optogenetics-LED-Violet spectrum                  Optogenetics-LED-Violet Rise/Fall Time.
     Ch1 – TTL input , Ch2 Photodiode measurement


Electrical Specifications

Digital modulation input   Optically isolated TTL
Connector for TTL and Analog input   BNC
Digital modulation frequency Hz DC-30000
Rise / Fall time (10% - 90%) μs >3/ >10
Analog input voltage range V 0-5
Input Voltage V 12
Power Adaptor Input   85-264 VAC, 47-63Hz,1.5A
 


General Specifications

Operation temperature range °C 10-35
Storage Temperature range °C -10-35
Operating relative humidity
Non condensing
% <90
Dimensions (L x W x H) mm 197 x 174 x 80
Weight g 750
Power Adaptor Dimensions
(L x W x H)
mm 175 x 72 x 35
Power Adaptor Weight g 270
Power Adaptor Safety  
Fan Noise dBA 28.4


 For more details on optional accessories please see:

Please refer to the in-vivo Optogenetics system diagram displayed above.

Rotary Joint [2]:

Prizmatix's Rotary Joint has very low torque and friction, with minimal impact on behavior. Prizmatix's Rotary Joint is specially designed for Optogenetics experiments with High NA detachable fibers equipped with FC connectors. Rotary Joint can be used with single or multiple output fibers simultaneously, enabling concurrent delivery of light to separate areas of the brain without loss of power or brightness. For more details please see:
https://www.prizmatix.com/optogenetics/rotary-joint.htm


Fiber Optic Cannulae [3]:

Prizmatix Implantable Fiberoptic Cannulae / Ferrules allow direct light stimulation of certain deep brain regions in living animals. Each Cannula (implant) consists of a zirconia ferrule accommodating a high NA Silica/POF fiber, protruding from the zirconia ferrule at the specified length. For more details please see:
https://www.prizmatix.com/optogenetics/OG-Cannulae.aspx


 Single or Dual Fiber for bilateral activation [4] and Fiber Patch
 cords [5]

Prizmatix provides diverse fiber optics solutions for Optogenetics in-vivo. Made of silica, glass or polymer, these high NA fibers can be constructed to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. Examples include single stainless steel coverings of bare tipped fibers, or Y-shaped fibers for simultaneous stimulation of two hemispheres. For more details please see:
https://www.prizmatix.com/optogenetics/Fiberoptics-for-Optogenetics.htm


 Pulser module with Windows software [6]:

The Pulser - USB pulse train generator with user friendly software is an easy way to generate trains of pulses for Optogenetics activation directly from your computer. The Trigger-In input enables synchronization of Optogenetics activation with various experimental events.
For more details please see:   https://www.prizmatix.com/optogenetics/PulserPlus.htm

© Prizmatix, Israel