“The strong output of UHP-Mic-LED-460 solved
this problem very effectively. As a bonus, the
simple control of its LED via TTL signals also
helped us to simplify synchronization of our
optical and electrophysiological instruments.”
Because they were photostimulating multiple neurons,
it was challenging to get enough light power to efficiently activate each neuron.
“The strong output of UHP-Mic-LED-460 solved this problem very effectively,” Dr. Tsuda said.
“As a bonus, the simple control of its LED via TTL signals also helped us to
simplify synchronization of our optical and electrophysiological instruments.”
The Prizmatix UHP-Mic-LED-460 provides more than 1.5 W of collimated light,
with a peak excitation of 460 nm, and is optimal for photostimulating a large number of neurons.
Prizmatix LEDs all feature a direct TTL input for fast switching with a rise/fall time of microseconds,
much faster than the millisecond pulses required for optogenetic applications.
As a proof-of-principle, the researchers used their technique to control the activity of cerebellar
interneurons while simultaneously recording inhibitory responses in multiple Purkinje neurons, which
are the postsynaptic targets for the interneurons. The results demonstrated that their all-optical
technique allows rapid and quantitative analysis of the spatial organization of neuronal circuits.
“We believe that this approach will greatly aid understanding of the functional organization
of neuronal circuits,” Dr. Tsuda said.
The researchers are now working to increase the resolution of photostimulation mapping by
optimizing the detection of neuronal responses, and they plan to try the approach with genetically
encoded fluorescent indicators to achieve cell-type specificity for the neuronal responses.
Dr. Tsuda adds that their approach could be used to analyze neuronal circuits in intact brains,
and for such in vivo applications, the UHP-Mic-LED-460 would be a key component because
of its high power and simple control.
Research Paper: Tsuda S, Kee MZ, Cunha C, Kim J, Yan P, Loew LM, Augustine GJ.. Probing the
function of neuronal populations: combining micromirror-based optogenetic photostimulation
with voltage-sensitive dye imaging.
Neurosci Res. 2013 Jan;75(1):76-81
UHP-LED for patterned illumination
Prizmatix Products for Research
In this research Dr. Tsuda used Prizmatix Ultra High Power Collimated LED (P/N: UHP-Mic-LED-460),
the best product for year 2013. Since then we improved UHP product lines and now Prizmatix offers more advanced models.
Currently main product lines are:
UHP-T-MP – Most powerful collimated LED with rectangular shape LED emitter (about 3x4mm).
Providing rectangular beam for applications such as microplate illumination.
UHP-T-DI - Powerful collimated LED with square shape LED emitter (3x3mm).
Providing square beam for application such as epifluorescence microscopy,
petri dish illumination, optional 5mm core light guide coupling.
UHP-T-EP – Powerful and high brightness collimated LED with square shape LED emitter (2x2mm).
Providing square beam for application such as epifluorescence microscopy, DMD, Optogenetics,
optional 3mm core light guide coupling.
UHP-T-SR – Ultra Bright collimated LED with small LED emitter (several sizes available).
Providing extra brightness for application such as epifluorescence microscopy, Optogenetics,
optional 3mm core light guide coupling and fiber coupling.
Advantages of UHP-T series:
Location of high current LED driver
In UHP-T-LED the high current LED driver is located inside LED head rather than in controller.
In similar products from other vendors the high current driver located in the controller box.
When high current flows from controller to LED head high RFI/EMI interference
may disturb delicate measurements. In UHP-T-LED models the LED head is grounded and
serve as Faraday cage reducing the RFI/EMI interference.
Optically Isolated TTL and Analog Inputs
The controller of UHP-T-LED features, as standard, TTL input for very fast triggering
(or ON/OFF strobing) of LED light without need of a shutter. The Analog input provides
simple way to control the LED light power from computer. Both inputs are independent
and optically isolated to eliminate ground loops.
Low Optical Noise Option
Most modules can be purchased with Low Noise (-LN) option. The low optical noise
light source is very important in experiments involving such measurements as
cell membrane potential imaging by Voltage-Sensitive Dyes (VSD) (potentiometric dyes).
In many preparations the change of Voltage-Sensitive Dye fluorescence signal may be
just few percent. If the excitation light source exhibits high intensity fluctuations
the small VSD changes may be obscured. UHP-T-LED-LN has intensity fluctuations of
less than 0.01% RMS between DC to 1MHz, enabling detection of small changes in Voltage-Sensitive Dye