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The UHP-T-MP series Ultra High Power LEDs (~50 Watt) feature a powerful rectangular (3:4) collimated LED beam (direct illumination without a light guide) useful for direct illumination of biological Well Plates (such as for example 96 well microplate). These Collimated LEDs are excellent for such power demanding applications as Optogenetics with Channelrhodopsin (ChR2), ArchT, Halorhodopsin (NpHR) and other Opsins, Photoactivation, Uncaging, Photobleaching and Photochemistry.

The UHP-T-MP optics contains aspheric lens to create collimated LED beam with almost flat field illumination of standard 96 well microplate from working distance of about 40cm (see example of beam profile).

The LED current controller supports CW or pulsed operation via TTL trigger. The LED power can be controlled by 10 turn dial or via Analog input (0-5V). The UHP-T-MP LEDs are available in blue, green and red wavelengths.

The product can be used in various configurations such as collimated light source, directly connected to microscope, coupled to liquid light guide or coupled to optical fiber. Multi-wavelength light source can be easily created by several UHP-T units combined by a Beam Combiner.

Please see full list of optional accessories below.

UHP-T-MP LED


Click picture to enlarge

Click picture to enlarge


Key Features:

  • Single chip Ultra High Power LED 3:4 aspect ratio.
  • Close to Flat Field Collimated beam.
  • Optically isolated TTL input for external triggering (no shutter needed) or strobe operation.
  • Optically isolated Analog input (0-5V) for LED power control by external device like D/A interface.
  • Computer control via USB by Windows software, LabView VI or uManager (optional)
  • Compatible with Prizmatix modular Microscope-LED Light Source products family - see below for details
  • LED spectrum can be narrowed by band pass filters or a filter wheel.

 

Prizmatix LEDs modular versatility

  

Applications:

  • Microplate illumination, Petri dish illumination.
  • Optogenetics, Photoactivation, Uncaging, Photobleaching and Photochemistry.
  • Whole body imaging of small animals.
  • Bio analysis.
  • Machine Vision.
  • OEM


Click to enlarge

Optical Specifications

Product P/N Total Power [mW] Normalized Value [a.u.] Bandwidth Power[mW]


Center [nm] Width [nm]

a.u.
μW/nm
Wavelength (nm)  


 Beam Profile Measurements

Remarks:

 Benchtop LED Current Controller Specifications

  • Constant current or chopping modes
  • Precise LED current setting by 10 turn dial
  • TTL external trigger input
  • Analog input for external LED power control (0-5Vdc)
  • Optically isolated TTL and Analog inputs
  • Compact and robust enclosure
Click to enlarge


Electrical Specifications:

Connectors for TTL and Analog Input   Optically isolated BNC
Digital modulation frequency Hz DC-10000
Rise / Fall time μs <10/<3
Analog input voltage range V 0-5
Analog modulation frequency Hz DC-~100
Current controller supply voltage V 12
Power adaptor input   85-264 VAC, 47-63Hz, 1.5A

General Specifications:

Operation temperature range °C 10-35
Storage temperature range 0C -10-55
Operative relative humidity (Non condensing) % <90
Dimensions See drawing below
Head weight g 350
Controller dimensions (L x W x H) mm 197 x 174 x 80
Controller Weight g 400
Power adaptor dimensions
(L x W x H)
mm 175 x 72 x 35
Power adaptor weight g 650
Power adaptor safety  
LED Head fan noise dBA 38

Mechanical Drawings


Standard UHP-T LED head:

UHP-T-White-Drawing

When application requires prolonged illumination periods in continuous mode the cooling fan may be activated and disturb the imaging. In such cases please consider detached fan option as described in the drawing below:

Hose

  * Specifications subject to changes without notice


Optional Accessories

For full details on optional accessories please see:

https://www.prizmatix.com/optogenetics/Optogenetics-LED-Light-Sources-and-Fiber-Optics.htm

Accessories
 

All Prizmatix's LED light sources can be easily configured for use in a wide variety of applications by simply adding further components. These use a standard 1.035″ 40TPI thread (SM1 compatible) or Prizmatix's 4-pin connection system.

The following table summarizes the most popular accessories according to application, further information on individual part can be found below the table. Custom solutions are also often available at a low cost: please contact us with your specific requirements.



Prizmatix Ultra High Power LED Light Source [1]

Any type of Prizmatix Ultra High Power LEDs can be combined to form multi-wavelength system. For more information on specific types please see following links:  Mic-LEDs,  UHP-LEDs.

Beam Combiner [2]

Beam Combiner module joins two discrete Prizmatix LEDs into one output beam using a built-in dichroic mirror at specified cut-off wavelength .
The beam combiner has additional port that can be used for connection of monitoring photodiode.

FCA - Fiber Coupler Adaptor [3]

All Prizmatix LED heads can be easily changed from collimated LED source to fiber coupled LED configuration by means of Fiber Coupler Adaptor (SMA, FC or ST connector). .
For more details please see video clip

Fiber patch cords [4]

Various fiber optic patch cords are available. Most popular patch cords are 1000 / 1500 / 2000
micron core diameter Polymer Optical Fibers (POF) terminated by optical SMA or FC connectors.
For Optogenetics applications Dual or Triple Y shape bundles can be useful for multi-site activation.
For spectroscopy application special fiber optic bundles are very important. Care must be addressed to eliminate possible cross link between the excitation and the collection fibers. See more info here.

Rotary-Joint [5]

Prizmatix Rotary Joint is especially designed for Optogenetics experiments with High NA fibers equipped with FC connectors. Due to very low friction and smooth swivel the rotary joint is suitable for use even with small mice. See more info...

Beam Switcher [6]

The Beam Switcher accessory allows for Prizmatix LEDs installed on a microscope to be used either as microscope epi-fluorescence illumination or illuminating a specimen via a fiberoptic probe this feature especially useful for in-situ Optogenetics studies. See more info...

Filter Wheel [7]

The manual 6 positions filter wheel can be especially useful for White LED sources. The Filter Wheel can be installed at the beam output of LED or a Beam Combiner. See more info...

Microscope Adaptors [8]

Adaptors enabling connection of Prizmatix LED light sources to epi-fluorescence light port. Adaptors for Nikon, Zeiss, Olympus and Leica microscopes are available. See more info...

LLG-A Liquid Light Guide Adaptor [9]

The LED system can be quickly and easily attached to Liquid Light Guide by means of LLG-A Adaptor. For more details please see video clip

LLG-3 / LLG-5 [10]

The Liquid Light Guide is a common way to connect light source to microscope epi-fluorescence port. Prizmatix can provide 3mm or 5mm core LLGs.

LLG-C collimator XYZ [11]

The Prizmatix LLG collimator is an XYZ adjustable collimator for 3mm core Liquid Light Guide (LLG-3) with adaptor for the epi-illumination port of fluorescence microscopes. This collimator can be equipped with Nikon, Olympus, Zeiss or Leica adaptors. See more info...

C-Mount Adaptor [12]

A standard C-mount thread adaptor (1-32 UN 2A thread), usually used to connect to trinocular microscopes.

Collimator [13]

The output from optical fiber is divergent relative to fiber NA. In order to reduce the divergence angle a collimator module can be used. Prizmatix's collimator is especially designed to fit thick core high NA polymer optical fibers. See more info...

Reference Photodiode [14]

Prizmatix reference photodiode is useful in order to monitor LED source power over time in experiments required exceptional stability over long time period. See more..

Optogenetics-Fiber for in-vivo [15]

Prizmatix provides a full solution to the optogenetics in-vivo and in-vitro fiber optics. Made of silica or polymer high NA fibers, the high NA fibers are assembled to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. This is example of a Y-shaped fiber for simultaneous in-vivo stimulation of two hemispheres. See more info...

Implantable Cannulae [16]

Prizmatix Implantable Fiberoptic Cannulae / Ferrules allow direct light stimulation of certain deep brain regions in living animals. Each Cannula consists of a Zirconia ferrule accommodating a high NA Silica optical fiber, protruding from the Zirconia ferrule at a desired length. See more info...

Optogenetics-Fiber for in-vitro [17]

Prizmatix provides a full solution to the optogenetics in-vivo and in-vitro fiber optics. Made of silica or polymer fibers, the high NA fibers are assembled to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. This is example of a fiber with stainless steel tip with protruding bare fiber for in-vitro activation of brain slices under a microscope. See more info...

Pulser [18]

The Pulser USB to TTL interface box with user friendly software is an easy way to generate trains of pulses for Optogenetics activation directly from your computer. See more info...

USB-TTL Interface [19]

The microprocessor based device enabling simple control of Prizmatix LED sources from Windows based computer via USB.

Application Examples:

Optogenetics LED Light Source:

Optogenetics illumination sources include lasers and LEDs, and photoactivation can be done under a microscope or via a fiber for in-vitro or in-vivo applications. Using a Prizmatix LED illumination system for optogenetics studies provides many advantages compared to laser-based systems.
Read more... 


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